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| Carvalho,Otieres Cirino de; Paiva Neto,Vespasiano Borges de; Padilha,Daly Roxana Castro; Veloso,Tomás Gomes Reis; Bocayuva,Melissa Faust; Soares,Deanna Carla Oliveira; Kasuya,Maria Catarina Megumi. |
| ABSTRACT Symbiosis between orchid seeds and mycorrhizal fungi has been reported to be a determining factor in the success of germination and protocorm development in vitro. The aim of this study was to isolate and identify by molecular analysis the mycorrhizal fungus associated with Cyrtopodium paludicolum, and to evaluate its efficiency in facilitating seed germination and development. Germination experiments were carried out using a fungus isolated from C. paludicolum (CH01) and Epidendrum secundum (M65), which has been successfully used a number of times in symbiotic germination. The experiments were conducted in a completely randomized design with treatments of CH01, M65 as well as under asymbiotic conditions. The mycobiont CH01 was successfully... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Cerrado; ITS-DNAr; Mycorrhiza; Orchidaceae; Symbiosis. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000100107 |
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| Paiva Neto,Vespasiano Borges de; Campos,Guilherme de Oliveira; Boaretto,Amanda Galdi; Zuffo,Monica Cristina Rezende; Torrezan,Mateus de Aguiar; Benetão,Jamile. |
| Aspasia variegata occurs naturally in the savanna of the Mato Grosso do Sul State, Brazil and it has been widely collected for its beautiful flowers. Additionally, its habitat has been greatly reduced and little or no investigation of its spread has been performed. Aiming to establish a protocol to obtain seedlings of the orchid A. variegata, different compositions of culture medium were tested to identify which one provided better in vitro growth and development and to assess the influence of these media in seedling acclimatisation. Thus, seeds obtained from mature capsules were inoculated in Knudson culture medium for 120 days until the protocorm stage. They were transferred to different culture media formulations, including MS and Knudson with half or... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Orchidaceae; Activated charcoal; Chlorophyll content; Acclimatisation. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013001200010 |
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| Paiva Neto,Vespasiano Borges de; Paiva,Renato; Furtado,Deborah E.. |
| With the objective to investigate the in vitro behavior of salix (Salix humboldtiana Willdenow), leaf and nodal segments were inoculated in WPM medium supplemented with NAA and IBA. In leaf explants, the presence of these growth regulators, isolated or in combination, induced the formation of adventicious roots. Root hairs were only observed when NAA was present. In nodal explants, while the combination of 2,68 µM ANA + 2,46 µM IBA induced bud growth, the other treatments (4,92 µM IBA; 2,68µM ANA + 4,92 µM IBA; 5,37 µM ANA + 4,92 µM IBA; 5,37µM ANA) induced the formation of adventicious roots. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Tissue culture; Salix; Adventicious roots; Root hairs. |
| Ano: 1998 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89131998000100009 |
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